Western Blot Temel Sarf Listesi
Protein İzolasyonu | RIPA Lysis Buffer |
Protease/Phosphotase Inhibitör Kiti | |
Protein Elektroforezi | 4-12% Tris-Glycine Gel 1.0 mm, 10 göz |
2x Laemli Loading Buffer | |
Tris-Glycine SDS Running Buffer | |
Pre-stained Protein Standard | |
ECL Pre-Stained Protein Standard | |
Western Blot | Nitroselüloz veya PVDF Membran |
Blotlama Kağıdı | |
Tris-Glycine Transfer Buffer | |
Membran Boyama | Ponceau-S, Sodium Salt |
Deteksiyon
(Kemilüminesan Görüntüleme) |
BSA |
TBST | |
Sekonder Antikor | |
Primer Antikor | |
ECL/Luminol Reagent vb |